Bulk transcriptomic profiling of eosinophils from skin and bone marrow during atopic dermatitis

Atopic dermatitis was induced on dorsal skin of 7-10 week-old C57BL/6 female mice by bi- or tri-daily MC903 treatment. On day 8, viable mouse eosinophils (CD45+F4/80+SiglecF+) were sorted from skin regions of healthy or vehicle/treated atopic dermatitis or femur/tibia-derived bone marrow by FACS. Samples were paired within individual mice (4 mice each). RNA from 500 cells were isolated and profile for transcriptome by sequencing. Overall design: Dermal and bone marrow eosinophils were sorted by surface antigens by flow cytometry followed by transcriptomic profiling with bulk RNA-seq.