bivariate flow graph of maize starch

Using cytometry to separate starch fractions according to granule size , fine structure and particle properties reflected by FSC (forward scatter) and SSC (side scatter). To prove the flow analysis caused by the real property of subpopulations rather than an optical artifact. The specific parameters were FSC (79-143), SSC (201-223) and FITC (257-425) and FSC (129-255), SSC (192-237) and FITC (267-417) for the first group and second group respectively.