Viability of adhered cbHSPCs after 72 hours in co-culture with ASCs.
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To evaluate the viability of attached cbHSPCs, ASC/cbHSPC co-cultures were trypsinised, and the cell suspension was stained with the Annexin V—FITC/PI kit according to the manufacturer’s instructions, before being analysed on an Epics XL flow cytometer. For analysis, only cells gated as CD45+/CD90- were used. The data are presented as M+S.E.M. (n = 4).Viability of adhered cbHSPCs after 72 hours in co-culture with ASCs.
创建时间：
2015-12-03
