Role of 3-Hydroxyisobutyrate in Cardiometabolic Disease

The valine catabolite 3-hydroxyisobutyrate (3HIB) was shown to promote insulin resistance in skeletal muscle by increasing lipid content in vivo. Translating this finding to cardiovascular system, it might be hypothesized that 3HIB deranges trans-endothelial fatty acid transport with potential clinical implications. Methods: To measure the impact of 3HIB on the development and progression of atherosclerotic lesions in vivo, ApoE-/- mice were fed a standard laboratory diet (SD) or a Western Diet (WD) for 12 weeks starting at 8 weeks of age to induce atherosclerosis, in presence or not of 3HIB (300mg/kg, i.p injected 3 times a week) (n=6 per group).