Stella prevents excessive de novo DNA methylation during mouse oogenesis

Oocyte acquires developmental competence during its maturation. This stage is accompanied with large-scale alteration in transcription, and series of genome-wide epigenetic reprogramming, including de novo establishment of DNA methylation. However, our understanding of mechanisms regulating this process is limited. To investigate the role of Stella (Dppa3) in de novo methylation during mouse oogenesis, here we measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages, including genotypes of both Stella (Dppa3) +/- and Stella -/-. We measured DNA methylation by RRBS and expression profiles by RNA-seq in PGCs and oocytes at serveral development stages (E13.5, D5, D10, D20, MII), including genotypes of both Stella (Dppa3) +/- and Stella -/-. DNA methylation of sperm with both genotypes were also measured by RRBS for comparision.