Data from: Novel microsatellite markers for epiphytic bromeliad Tillandsia recurvata L., in an urban landscape in South-eastern Brazil

The authors present seven novel microsatellite markers for Tillandsia
recurvata L. The genome assemble sequences of T. recurvata were obtained
from NCBI (7.2Gb) (Sayers et al. 2022). The BioProject Accession and
accession numbers are PRJNA701548 and SRX10089449, respectively. The
microsatellite identification software Krait (0.5.2) (Du et al. 2018) was
used to detect suitable microsatellites, both genome-wide and in the
noncoding regions of T. recurvata. The single sequence repeats (SSRs) were
refined to a minimum number of seven repeats of di-, tri-, or
tetra-nucleotide repeat motifs. These sequences were further limited to
SSRs of more than 100bp in length and low GC content (<50%). Krait
(0.5.2) (Du et al. 2018) was also used to design the primers for the
selected SSR sequences, in conjunction with the integrated Primer3
software. The criteria for primer selection included: a primer length of
18-26bp, an optimal melting temperature (Tm) of 54-59°C and GC content of
<50%. These designed primers were single-plexed and amplified using
the following PCR cycle: initial denaturation (95°C for 3 min), 34 cycles
of 95°C for 30s, annealing for 30s (JP01-JP12: 54°C, 4873TD + 35251TD:
56°C, 19286TD: 53°C, 5044TD + 186664TD + 214633TD: 58°C), 72°C for 1 min
and a final extension of 72°C for 5 mins. The authors make this
information available to other researchers, to continue the investigation
of epiphyte genetics.