Stat6 occupancy profiling by high throughput sequencing from wild-type(WT) or Trim24-deficient(Trim24-KO) mouse peritoneal macrophages stimulated with IL-4 for 1 hour .

To identify the potential genes that regulated by Trim24 through Stat6 DNA binding activity, we immunoprecipitated chromatin from WT or Trim24-KO macrophages stimulated with IL-4, and analyzed the precipitated DNA with deep sequencing (ChIP-Seq). To exam the difference of Stat6 DNA binding in WT and Trim24-KO macrophages,the chromatin of mouse macrophages stimulated for 1 hour with IL-4 was prepared and followed by ChIP-Seq analysis by Active Motif, Inc. using the antibody against Stat6.