Saccharomyces cerevisiae W303. Saccharomyces cerevisiae W303

Chromosome replication in Saccharomyces cerevisiae is initiated from roughly 300 origins that are regulated both by DNA sequence and by the limited abundance of four trans-acting initiation proteins (Sld2, Sld3, Dpb11 and Dbf4, collectively called “SSDD”). We set out to determine how the association of Sld2 or Sld3 at origins contributes to time of origin activation and/or origin efficiency by using auxin-induced protein degradation to further decrease their abundance. This study provides insights both into the contribution of essential initiation factors to genome stability and into the interplay between origin efficiency and timing.