BIOGRID CURATED DATA FOR PUBLICATION: A DNA integrity network in the yeast Saccharomyces cerevisiae.

Protein-Protein, Genetic, and Chemical Interactions for Pan X (2006):A DNA integrity network in the yeast Saccharomyces cerevisiae. curated by BioGRID (https://thebiogrid.org); ABSTRACT: A network governing DNA integrity was identified in yeast by a global genetic analysis of synthetic fitness or lethality defect (SFL) interactions. Within this network, 16 functional modules or minipathways were defined based on patterns of global SFL interactions. Modules or genes involved in DNA replication, DNA-replication checkpoint (DRC) signaling, and oxidative stress response were identified as the major guardians against lethal spontaneous DNA damage, efficient repair of which requires the functions of the DNA-damage checkpoint signaling and multiple DNA-repair pathways. This genome-wide genetic interaction network also identified novel components (DIA2, NPT1, HST3, HST4, and the CSM1 module) that potentially contribute to mitotic DNA replication and genomic stability and revealed novel functions of well-studied genes (the CTF18 module) in DRC signaling. This network will guide more detailed characterization of mechanisms governing DNA integrity in yeast and other organisms.

蛋白质-蛋白质、遗传和化学相互作用数据集（Pan X，2006年）：酵母菌Saccharomyces cerevisiae的DNA完整性网络。由BioGRID（https://thebiogrid.org）编纂；摘要：通过全局遗传分析合成适应性或致死性缺陷（SFL）相互作用，在酵母中鉴定出一种调控DNA完整性的网络。在该网络中，基于全局SFL相互作用的模式定义了16个功能模块或亚途径。参与DNA复制、DNA复制检查点（DRC）信号传导和氧化应激反应的模块或基因被确认为对抗致死性自发DNA损伤的主要守卫者，其高效修复依赖于DNA损伤检查点信号传导和多个DNA修复途径的功能。该全基因组遗传相互作用网络还鉴定出可能参与有丝分裂DNA复制和基因组稳定性的新型组分（DIA2、NPT1、HST3、HST4以及CSM1模块），并揭示了在DRC信号传导中已研究深入的基因（CTF18模块）的新功能。该网络将指导对酵母及其他生物体中调控DNA完整性的机制进行更深入的表征。