HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest

This dataset supports the following findings: the HIV-1 Virion Infectivity Factor (Vif) promotes viral infection by targeting and degrading cellular APOBEC3 proteins, which are key regulators of intrinsic and innate antiviral immunity. Although Vif is also known to induce cell cycle arrest, its precise effects on the cell cycle have remained unclear. Using high-temporal-resolution single-cell live imaging and super-resolution microscopy, we found that Vif does not disrupt the G2/M boundary as previously proposed. Instead, Vif induces a distinct and robust pseudo-metaphase arrest, unlike the mild prometaphase arrest caused by Vpr. During this arrest, chromosomes initially align at the metaphase plate but later lose alignment, producing polar chromosomes. Notably, Vif markedly reduces Protein Phosphatase 1 (PP1) and Protein Phosphatase 2A (PP2A) levels at kinetochores, unlike Vpr. These findings reveal a novel role for Vif in kinetochore regulation and chromosome organization during mitos..., , , # Data from: HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest [https://doi.org/10.5061/dryad.k6djh9wj7](https://doi.org/10.5061/dryad.k6djh9wj7) ## Description of the data and file structure Raw datasets related to the manuscript (Ghone et al., 2025, HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest. *eLife***13**:RP101136). These source datasets were used to generate the figures presented in Ghone et al (2025) \"HIV-1 Vif disrupts phosphatase feedback regulation at the kinetochore, leading to a pronounced pseudo-metaphase arrest\", eLife (eLife-VOR-RA-2024-101136R1). The original article is fully and freely available to the public. Readers are encouraged to review the article together with these datasets, as the labels, variables, and definitions in the datasets correspond directly to the figures. Each dataset also provides figure numbers for conve..., ,