RNA binding proteins (RBPs) perform a myriad of functions and are implicated in numerous neurological diseases. To identify the targets of RBPs in small numbers of cells, we developed TRIBE, in which
All concentrations are given per reaction. n.p. qPCR was not performed. a5-virus-mix consists of the HIV-1 strains JR-CSF, NL4-3, HXB2, YU2 and 89.6 (see also Materials and Methods). b3/23 µl of cDNA