File S1 - Targeting NAD+ Metabolism in the Human Malaria Parasite Plasmodium falciparum

Contains the files: Figure S1. PfNico (PFC0910w) localization throughout the IDC. Live imaging of episomally expressed enzyme-GFP fusion proteins in the parasite. Figure S2. Labeling pattern of NAD+. The resulting labeling pattern of NAD+ from C13-U-glucose is depicted. Figure S3. Observed labeling pattern of NAD+ in iRBCs. Comparison of half and full labeled NAD+ generated in iRBCs in the presence of C13-U-glucose. Figure S4. NAD+ synthesis under different niacin conditions. Observed fold change in NAD+ concentration resulting from different niacin present in the culture medium. Figure S5. Alignment of PfNMNAT and the E. coli homolog NadD. Comparison of the amino acid sequences of the parasite and bacterial NMNAT enzymes. Figure S6. Complementation of E. coli NadD with PfNMNAT. The ability of the parasite NMNAT to rescue E. coli growth is demonstrated in a continuous growth assay. Figure S7. Alignment of PfNMNAT and the Human NMNAT homologs. Comparison of the amino acid sequences of the parasite and human NMNAT enzymes. Table S1. Primers Used in This Study. Table S2. Strains Used in This Study. (PDF)