Transcriptome sequencing in Scutiger sikimmensis

<b>Data in Brief</b><br>Title: Transcriptome sequencing and the presence of high-altitude and sex-determination genes in <i>Scutiger sikimmensis</i><br>Authors: Sylvia Hofmann¹, Heiner Kuhl², Chitra Bahadur Baniya³, Matthias Stöck²<br><br>Affiliations<br>¹UFZ - Helmholtz-Centre for Environmental Research, Department of Conservation Biology, Permoserstrasse 15, 04318 Leipzig, Germany<br>²Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 301, 12587 Berlin, Germany<br>³Tribhuvan University, Central Department of Botany, Kirtipur, Kathmandu, Nepal<br><br><b>Abstract</b><br>The Himalayas are one of earth’s hotspots of biodiversity. However, modern evolutionary genomic studies in Himalayan vertebrates are still at the beginning. Moreover, in organisms, like most amphibians with relatively high DNA content, whole genome sequencing remains bioinformatically challenging and no complete nuclear genomes are available for Himalayan amphibians. Here, we present the first well-annotated multi-tissue transcriptome of a Himalayan lazy toad (Scutiger sikkimensis). Applying Illumina NextSeq 500 RNAseq to six tissues, we obtained 41.32 Gb of sequences, assembled to ~111,000 unigenes, and annotated them in seven functional databases. The annotation results and their statistics can be found in Supplementary Material S8 and S9. <br><br>Key words: Himalayan lazy toad; Trinity; RNAseq; Illumina; N50<br><b>Specifications</b> <br>Organism/cell line/tissue: <i>Scutiger sikkimensis</i>/brain, heart, liver, lung, kidney, testis tissue<br>Sex: male<br>Sequencer or array type: Illumina NextSeq 500<br>NCBI Project ID: PRJNA532534<br>Accession numbers: SAMN11403593-SAMN11403598SRA numbers: SRR9953599-SRR9953604<br>Experimental factors: De novo transcriptome assembly of <i>Scutiger sikkimensis</i><br>Experimental features: Tissue samples of a male specimen of the lazy toad<i> Scutiger sikkimensis</i> was collected for total RNA isolations. Prepared a paired-end library, sequenced by the NextSeq 500 system. The obtained data was subjected to de novo assembly.<br>Consent: N/A<br>Sample source location: Central Himalaya, Nepal<br>