Ceramides drive colorectal cancer metastasis through a revival stemness program [BulkRNAseq_CRISPR]

We demonstrate that tumor-intrinsic ceramide metabolism is crucial in initiating colorectal cancer (CRC) metastasis, a process further enhanced by dietary fat intake Overall design: The ibonucleoprotein CRISPR protoco was used to generate Degs1 knockout organoids. shYAP1 was used to generate YAP knockdown organoids. RNA was extracted on to check the expression changes after Degs1-KO and YAP1-KD.