Effect of statistical methods on the prediction of essential genes based on two datasets.

The predictive value of each method was assessed using ROC curves and a Welch T-test.*Cut-offs for S. pneumoniae R6 were automatically detected by ESSENTIALS while for P. aeruginosa PAO1 a cut-off of 2.5 fold underrepresentation of reads per gene in the challenge condition was used to facilitate comparison with the literature data from Gallagher et al.