Interaction pattern of ULD1 with DNA GyrB and ParE, and the position of ULD1 resistance-conferring mutations.

Table shows the position of first-step ULD1 resistance-conferring mutations within the drug’s binding pocket in GyrB and ParE at amino acid positions that are interacting with ULD1 (based on Induced Fit Docking and subsequent molecular dynamics simulation). Mutations have not been detected at interacting amino acids Asp81, Arg84, and Pro87 (in bold), in accordance with a prior study that has revealed that these residues are difficult to mutate as they have essential role in enzymatic function [39]. GyrB, subunit B of DNA gyrase; ParE, subunit E of topoisomerase IV. (XLSX)