RNAseq analysis of whole islets from pre-weaning wild type and Akita mice

This study aims to determine the effects of proinsulin misfolding/ER stress on ß-cell dynamics and function in Akita mice. The RNAseq experiment offers the opportunity to investigate the impact of proinsulin misfolding/ ER stress on the genetic signature of islets isolated during the neonatal period in the Akita mice preceding the development of hyperglycemia. Overall design: P19-21 Akita and age-matched control mice (n=3 in each group, each sample is pooling of islets from 3 mice) were isolated and processed for RNA exctraction. RNA sequencing libraries were then constructed from 120 ng of total RNA.