The role of glaciations in the evolutionary history of a widely distributed Neotropical open habitat bird

Aim: The Neotropics constitute the most biodiverse region of the world, yet its patterns of diversification and speciation differ among Neotropical areas and are not equally well understood. Particularly, avian evolutionary processes are understudied in the open habitats of temperate South America, where the role of glacial cycles is not clear. We analyzed the evolutionary history of a Neotropical widespread bird species as a case study to evaluate its continental-scale patterns and processes of diversification, with a focus on Patagonia.
Location: Open habitats of the Neotropics.
Taxon: Vanellus chilensis (Aves, Charadriiformes).
Methods: We obtained reduced representation genomic and mitochondrial data from the four subspecies of V. chilensis to perform a phylogenetic/phylogeographic analysis and study the evolutionary history of the species. We complemented these analyses with the study of vocalizations, a reproductive signal in birds.
Results: The initial diversification event withi..., We performed double-digest restriction site-associated DNA sequencing (ddRADseq; Peterson et al., 2012) following the protocol detailed in Thrasher et al. (2018). Genomic DNA from fresh tissue and blood samples was isolated using the DNeasy tissue extraction kit (Qiagen, CA, USA). For each individual, we isolated ~500 ng of genomic DNA at a standardized concentration of 20 ng/µl (quantified using a Qubit fluorometer; Life Technologies, NY, USA). DNA was digested with the restriction enzymes SbfI High Fidelity (8 base bp recognition site; 5’-CCTGCAGG-3’) and MspI (4 base bp recognition site; 5’-CCGG-3’) (New England BioLabs, MA, USA). The 5' and 3' ends of the digested genomic DNA were ligated to P1 and P2 adapters, respectively. Sequences of these adapters are available in Peterson et al. (2012). Samples with unique P1 barcodes were pooled into different indexing groups postdigestion/ligation and cleaned up using 1.59 Agencourt AMPure XP beads (Beckman Coulter, CA, USA). Samples were or..., , Vanellus chilensis ddRAD data
\-\-\-
LOCI file containing the ddRAD data of 45 samples of the Southern Lapwing (Vanellus chilensis) collected in Brazil, Argentina, Uruguay and Chile.
Title of the manuscript: The role of the glaciations in the evolutionary history of a widely distributed Neotropical open habitat bird.
Authors: Belén Bukowski, Leonardo Campagna, María José Rodríguez-Cajarville, Gustavo S. Cabanne, Pablo L. Tubaro and Darío A. Lijtmaer.