The intestines from Litopenaeus vannamei for 16s sequencing Raw sequence reads

Soybean meal is considered as one of the major components in Litopenaeus vannamei diets. However, most previous studies have focused on evaluating the effects of soybean meal on from the perspective of growth, physiological and feed utilization, information regarding the analysis of serum metabolites, antioxidant and immune response and intestinal microbiota is limited. Five diets were formulated to contain 20% (T20), 28% (T28), 35% (T35), 42% (T42) and 50% (T50) of soybean meal. A total of 600 L. vannamei were randomly distributed into 20 tanks (30 shrimp per tank) with 4 tanks being assigned to each diet. Shrimp were fed to apparent satiation three times daily during the 42-d feeding trial. Results showed that serum globulin, alanine aminotransferase and aspartate aminotransferase were linearly increased (P < 0.01), but high density lipoprotein cholesterol was linearly decreased (P < 0.001) as dietary soybean meal increasing. Supplementation of soybean meal in shrimp diets linearly and quadratically increased (P < 0.05) serum total antioxidant capacity, catalase, malondialdehyde, nitric oxide synthase and phenoloxidase. Hepatocytes in T35, T42 and T50 were shown to have different degrees of vacuolar degeneration, hepatic corpuscle atrophy and starlike lumen lose. Dietary inclusion of soybean meal altered the composition of intestinal bacterial microbiota at phylum level especially increased the abundance of potentially pathogenic bacteria, whereas it had minimal impact on bacterial genera and had no significant influence on the bacterial diversity.