Deep sequencing of transcript levels of human embryonic stem cell derived mesothelium (epithelial and mesenchymal forms) and mesothelium from neonatal mouse E15.5 of the heart, lung, liver and gut and ChIP-seq data of the mesenchymal form of mesothelium

Mesothelium is a multipotent resident progenitor cell of the coelomic organs that functions in organogenesis, repair and possible regeneration. We used hESCs to generate mesothelium of the epithelial and mesenchymal forms. Mesenchymal derivatives of mesothelium have been previously reported to function in tissue repair by promoting and participating in angiogenesis and neovascularization. We uncovered that hESC-derived mesothelium of the mesenchymal form (MesoT) are multipotent and generate smooth muscle cells, endothelial cells and pericytes and self-assemble into vessel-like networks in vitro. MesoT cells contribute to nascent coronary vessels in the repair zone of mechanically damaged neonatal mouse hearts. MesoT cells seeded onto vascular scaffolds self-assemble into vasculature capable of supporting peripheral blood flow following transplantation. Our findings demostrate the potential utility of MesoT cells in tissue repair and vascular engineering applications.