Synaptic scaling regulates the transcriptome in hippocampal neurons. Synaptic scaling regulates the transcriptome in hippocampal neurons

To better comprehend how synaptic scaling affects the neuronal transcriptome, we used the whole genome microarray expression profiling in hippocampal cultures treated with AMPARs and synaptic NMDARs antagonists for 9h and 26h, or under control conditions. Gene ontology enrichment analysis showed altered transcripts associated with synaptic signalling and synaptic plasticity classes, including transcripts of several proteins already associated with synaptic scaling mechanisms. Overall design: The effects of chronic synaptic activity blockade in gene expression were analyzed in primary cultures of rat hippocampal neurons treated for 9h or 26h with AMPARs antagonist GYKI-52466 and synaptic NMDARs antagonist MK-801. Three independent experiments were performed at each time (9 or 26 hours) as independent biological replicates.