Supplementary Materials 1 from Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

Dataset belonging to "Biases in bulk: DNA metabarcoding of marine communities and the methodology involved" Molecular Ecology (2020). LM van der Loos & R Nijland.
In order to synthesise and do a ‘stock take’ of recent
approaches and methods used for DNA metabarcoding of
bulk samples, we conducted a systematic literature survey. We searched the
database Web of Science in April 2019 using the key words “DNA metabarcoding”,
“marine”, “benthos”, “fauna”, “biodiversity”, “meiofauna”, “macrofauna”,
“sediment”, “diet” and “hard substrate” in various combinations of 2-3 keywords,
resulting in 518 returned records. Duplicate papers were removed as well as those
that were not primary research articles, not published in peer-reviewed
journals and not written in English. Following initial filtering, a total of
133 studies were considered for future reading, with data extracted only from
papers that concerned (a) eukaryotic organisms; (b) organisms sampled in a
marine environment, and (c) DNA metabarcoding of bulk samples. After excluding all
studies that did not meet these criteria, 64 studies published between 2010 and
2019 were analysed. For each study, we extracted information about the sample
type (plankton, soft sediment, hard substrate, or stomach content/faeces), the
target species groups, sample pre-processing, the preservation method, the DNA
extraction method, the selected markers (target DNA region and primers), the
PCR thermal profile, the number of technical PCR replicates, and the sequencing
platform used. This data is presented in the current table (Supplementary Materials 1).