Distinct Intestinal Microbial Signatures Linked to Accelerated Biological Aging in People with HIV

Genome-wide DNA methylation profiling of colon and ileal biopsies, blood samples from people living with HIV on ART and their matched HIV-negative counterparts. Despite having similar chronological ages, PWH on ART exhibit accelerated biological aging in the colon, ileum, and blood, as measured by various epigenetic aging clocks, compared to HIV-negative controls. Investigating the relationship between microbial translocation and biological aging, PWH on ART had decreased levels of tight junction proteins in the colon and ileum, along with increased microbial translocation. Bisulfite converted DNA from PBMC, Colon, and Illeum tissue were hybridized to the Illumina MethylationEPIC Beadchip