RNA sequencing of inguinal white adipose tissue from seipin-deficient mice and appropriate controls

We generated Bscl2lox/lox mice X ERT2-Adipoq-CRE mice, allowing inducible seipin deletion in adipocytes upon tamoxifen addition (iATSKO). Indeed, a 5-day tamoxifen treatment led to a dramatic decrease in Bscl2 mRNA in the adipose tissue. To study the effects of seipin deficiency in vivo on adipose tissue homeostasis in an unbiased manner, we used 3'-signal recognition particle (SRP) RNA sequencing. Overall design: This experiment compare the gene expression of 4 groups of mice (Bscl2lox/lox; Bscl2lox/lox -ERT2-Adipoq-CRE; Bscl2lox/lox+ tamoxifen; Bscl2lox/lox -ERT2-Adipoq-CRE + Tamoxifen that we call iATSKO. We are folowing gene expression over time : 15 days, 30 days, and 90 days after the initiation of tamoxifen injection.