Global change of RNA expression upon silencing TET1 in PDX2 B-ALL cells

Here we report that TET1 has a critical role in B-ALL development. TET1-depleted cells delayed the onset of B-ALL. To investigate the mechanisms, we first generate the patient-derived xenograft cells (PDX2) and transduce with inducible-Cas9 and sgRNA targeting TET1. After treatment with doxycycline, we observe the expression of Cas9 and a depletion of TET1 protein. By using the high throughput sequencing RNA-seq, we check the potential targets of TET1 in B-ALL cells. Finally, we validate that CD72 and JCHAIN are two essential targets of TET1 signaling in B-ALL cells. Collect the PDX2-sgNS and PDX2-sgTET1 cells after treatment with doxycylcine for 6 days, and extract total RNA. Use 10 µg for next library preparation and sequencing.