DNA methylation in Syrian hamster female fetal cells (gestation day 13.5) exposed in vitro to 5-aza-2'deoxycytidine (5aCdR)

Purpose: To validate the reduced representation bisulfite sequencing (RRBS) methodology by comparing DNA methylation pattern in Syrian hamster fetal (SHF) primary cells to a SHF cell line (MT2) exposed to the DNA methyl transferase inhibitor,5aCdR. The MT2 cell line was originally derived from the expansion of a benzo[a]pyrene-induced morphologically transformed clone (Pickles et al 2016. Mutat.Res.Genet.Toxicol.Environ Mutagen. 802:50-58). Overall design: The DNA methylation profiles of untreated SHF cells (4 biological replicates) were compared to untreated MT2 cells (4 culture wells as technical replicates, 0.05% DMSO as vehicle) and three wells treated with 5aCdR (5uM, 48 h). All cells were confirmed female using a rapid XY qPCR assay.