MeRIP-Seq reveals the genome-wide maps of m6A modification on mRNA and lncRNA in OSCC

We report the application of Methylated RNA Immunoprecipitation with Next Generation Sequencing and R software package in SCC25 and HAEC. By analysis the m6A peak areas which decorated in the 3' untranslated regions of target functional mRNAs and the entire interval of lncRNA, after that use gene ontology enrichment analysis and pathway enrichment analysis to indicate the explicit correlation to transcription, DNA-templated and pathways, we detect a series of m6A modification in mRNAs and lncRNAs, and explore some key clues in pathways related to OSCC which can guide the further studies on OSCC tumorigenesis and potential targeting. Overall design: Examination of m6A modification in human aortic endothelial cells and human tongue phosphorus carcinoma cells.