Transcriptomic analysis of the Pseudomonas sp. AP3_22 exposed to the sodium dodecyl sulfate

Here we presented the detailed transcriptomic analysis for Pseudomonas sp. AP3_22, an effective sodium dodecyl sulfate degrader isolated from the soil sample from wastewater treatment plant, cultured in the presence of SDS to get the first insight in the global bacterial response toward Sthis anionic detergent. Our results suggest showed that although SDS could be used as a carbon source, in the first place it acts influence on integrity of the cell envelopes and causes global stress response together combined with cell wall modification and repair induction. These results suggest that the modulation of the membrane content composition is first adaptation step in a typical response to detergent exposure. As the second response to the sodium dodecyl sulfate the AP3_22 strain metabolism was shifted from the lipid biosynthesis to the lipid catabolism and the SDS degradation started. AP3_22 strain was precultured overnight in LB medium. Then washed with minimal medium and diluted in the fresh 0.1 LB medium with or without (the control sample) SDS (5 g/L) to the initial OD600=0.4 and cultured in 30°C with 140 rpm agitation. The samples for RNA isolation were collected at following time points: at the beginning (0 minutes) and after: 30 minutes; 60 minutes; and 150 minutes of the experiment. The experiment was made in triplicate (three biological replicates for control and three biological replicates for the sample).