PCR results validating the presence or absence of phage sequences in the original E. faecalis Symbioflor 1, polylysogenic Symbioflor 1, and bacterial isolates from animals infected with polylysogenic Symbioflor 1.

Pp1 to 7 represent the seven prophages from E. faecalis V583 ΔABC, + indicates that sequences of that prophage were found in the respective isolate by PCR. The results confirm that the inoculum strain E. faecalis Symbioflor 1 was stably transduced with prophages pp1, pp5 and pp7, while bacteria isolated from animals contained pp1, pp5, and pp7, only pp5, or only pp1 and pp7. All Symbioflor 1 strains contain pp2 (part of the core genome). Primers used are shown in S2 Table.PCR results validating the presence or absence of phage sequences in the original E. faecalis Symbioflor 1, polylysogenic Symbioflor 1, and bacterial isolates from animals infected with polylysogenic Symbioflor 1.