Transcription factor induction of vascular blood stem cell niches in vivo [scRNA-Seq.Embryo-Niche]

We report single cell gene expression data for FACS-isolated zebrafish kdrl(flk1):mCherry and mrc1a.125:GFP double positive cells collected from whole embryos at 72 hours post fertilization (hpf). Overall design: kdrl:mCherry and mrc1a.125:GFP double positive transgenic embryos were homogenized, filtered, and then 25,000 GFP+ cells were FACS sorted into PBS. 3,000 cells were then encapsulated using the inDrops method and libraries were prepared for sequencing.