The mechanism by which many nutraceuticals promote health is incompletely understood. Some act by inhibiting the proteasome. Nuclear factor erythroid 2 related factor 1 (NRF1) is a stress-protective t
We grew wild type and corresponding mutant abf1 and rap1 temperature sensitive yeast at 25 C, then for one hour after shifting to 37 C. We then prepared nucleosomal (mostly mononucleosomal) DNA and hy
