Transcriptomic insight into molecular processes of dorsal muscle remodeling during Microhyla fissipes metamorphosis

We generated 120 Gb of raw sequencing data (~10 Gb per sample) and catalogued the expression profiles of 55106 annotated unigenes.We identified numerous differentially expressed genes that exhibited distinct expression patterns, and tight junction that were highly differentiated between S40 and S45, as well as between S36 and S43, S45. And genes related to glycolysis and pancreatic secretion were significantly enrichement beweent S43 and S45, these genes have known or potential roles in defense response and were enriched in functional gene categories potentially responsible for cucumber resistance.These results indicated that there are two process during metamorphosis, first one from S36-S43 is the larvel muscle development and apopsist, and the second from S43-S45 is the adult muscle development. Therefore, they are appealing candidates for further investigation of the gene expression and associated regulatory mechanisms related to muscle remodeling during metamorphosis. Overall design: Microhyla fissipes dorsal muscle mRNA profiles of four different development stage during metamorphosis were generated by deep sequencing with three biological replicates, using Illumina HiSeq4000.