A next-generation sequencing approach uncovers viral transcripts incorporated in poxvirus virions
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA401971
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资源简介：
Cowpox virus (CPXV) IMV particles adapted to HEp-2 or Rat-2 cells were purified by sucrose and CsCl gradient ultracentrifugation. RNA outside of virus particles was removed, RNA extracted and the viral DNA digested prior to NGS analysis. Three protocols were compared to elucidate transcripts in CPXV particles: (I) RNase digestion, (II) no RNase digestion and (III) protease digestion prior to RNase digestion.
创建时间：
2017-09-07
