Parameter estimates and their uncertainty for effects of tetrac formulations on proliferation of cancer cells (all results from NONMEM).

MDAMB: MDA-MB-231; P50, P10, P90: median, 10% and 90% percentile from bootstrap runs.The IC50 estimates for nano-tetrac are hypothetical concentrations that assume all of the tetrac bound to the nanoparticle is available for binding to the integrin receptor.The bootstraps were run both with a rich sampling design (n = 50 datasets) and the sampling times used in the actual experiments (n = 100 datasets).aFixed to 0.0001 µM as it was estimated very low and the lowest studied concentration was 0.001 µM.bImaxR at time = 0 d; ImaxR decreases with time (ImaxR = ImaxR0 · e−kiR · time), possibly due to functional adaptation of cells or the presence of two or more subpopulations with different sensitivities to tetrac.cFixed for the bootstrap.-not applicable.