Re-introducing non-optimal synonymous codons into codon-optimized constructs enhances soluble recovery of recombinant proteins from Escherichia coli
收藏Figshare2019-04-01 更新2026-04-29 收录
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https://figshare.com/articles/dataset/Re-introducing_non-optimal_synonymous_codons_into_codon-optimized_constructs_enhances_soluble_recovery_of_recombinant_proteins_from_Escherichia_coli/7932113
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The associated data files relating to the assessment of various molecular approaches to increase soluble expression in Escherichia coli. Sequences of codon optimised cDNAs used for the expression of recombinant small GTPases KRas 4B, RalA, and Rac1 are presented and positions selected for substitution with non-optimal synonymous codons are highlighted in red. In addition, the attached files contains tabulated data reporting the protein yields per litre for each replicate experiment from both soluble and denaturing IMAC purifications.
创建时间：
2019-04-01
