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RNA-guided Location and Function of the Cas9 CRISPR Endonuclease in Mammalian Cells

ChEBI2014-08-29 更新2026-07-21 收录

CRISPR-Cas9基因编辑

哺乳动物细胞功能定位

数据链接：

https://www.ebi.ac.uk/biostudies/ArrayExpress/studies/E-GEOD-57650 数据链接链接失效反馈

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资源简介：

RNA-guided Location and Function of the Cas9 CRISPR Endonuclease in Mammalian Cells

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创建时间：

2014-08-29

CRISPR-Cas9基因编辑

sgRNA优化

The current commonly used single-guide RNA (sgRNA) structure has a shortened duplex compared with the native bacterial clustered regularly interspaced short palindromic repeats RNA (crRNA)–transactiva

NIAID Data Ecosystem110

Target-bound SpCas9 complex with TRAC full RNA guide

CRISPR-Cas9基因编辑

结构生物学

Target-bound SpCas9 complex with TRAC full RNA guide

NIAID Data Ecosystem50

Application of iGUIDE to identify potential off-target cleavage caused by Cas9/PD1 sgRNA

CRISPR-Cas9基因编辑

CAR-T细胞疗法

We successfully develop a two-in-one approach to generate non-viral genome specific targeted CAR T cells through CRISPRCas9. In the adoptive therapy for relapsedrefractory aggressive B-cell non-Hodgki

NIAID Data Ecosystem40

Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation

CRISPR-Cas9基因编辑

小鼠遗传模型分析

Table S1. The guide RNA and singlestranded Oligonucleotide DNA sequences, their concentrations and the length of genomic regions floxed (in bp) reportedÂ in this study. (XLSX 29 kb)

Figshare2024-02-15 更新80

Engineering Tripartite Gene Editing Machinery for Highly Efficient Non-Viral Targeted Genome Integration

CRISPR-Cas9基因编辑

非病毒靶向基因组整合

We developed Cas9 fusion proteins containing single-stranded DNA-binding proteins and a 20-amino acid motif with single-stranded DNA-binding function. These fusion proteins demonstrated higher efficie

NIAID Data Ecosystem40

RNA-guided Location and Function of the Cas9 CRISPR Endonuclease in Mammalian Cells

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