Dataset for "NAMPT haploinsufficiency is a therapeutic vulnerability to NAMPT inhibition in -7/-7q MDS"

Dataset for article "NAMPT haploinsufficiency is a therapeutic vulnerability to NAMPT inhibition in -7/-7q MDS" Biomarker research, DOI: 10.1186/s40364-026-00956-6 Raw RNA sequening counts data for 30 MDS patient samples and 6 healthy samples. Total RNA was extracted from MNCs using the Qiagen miRNeasy kit or the Qiagen AllPrep kit. RNA quantification was done using the Qubit fluorometer (Thermo Fisher Scientific). Samples were sent for RNA sequencing in two batches. The first batch was sequenced by FIMM as follows: library preparation from 5 ng of total RNA was performed according to Takara SMARTseq v4 Ultra-low input RNA kit for Sequencing user manual (Takara Bio) followed by Illumina Nextera XT Library preparation according to Illumina Nextera XT Reference Guide (Illumina). Samples were sequenced on the Illumina NovaSeq6000 system using SP flow cell (Illumina) with read length for the single-end run was 75 bp. The second batch was sequenced by Biomedicum Functional Genomics Unit as follows: library preparation from 2 ng of RNA was done with SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing according to the user manual, followed by NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina. Samples were sequenced using NextSeq High Output with read length 75 bp. Reads were aligned to GRCh38.p13.