Hnrnpk is essential for embryonic limb bud development as a transcription activator and insulator collaborator

Proper development of limb bud relies on the concordance of various signals, otherwise limb deformities occur. We report that heterogeneous nuclear ribonucleoprotein K (hnRNPK) is essential for limb bud development. here, we knock out Hnrnpk in limb bud and exert the RNA-seq, ATAC-seq, CUT&RUN-seq, and Hi-C assay using primary limb bud cells to explore the function of Hnrnpk in limb bud development. ATAC-seq, mRNA, Ctcf binding, Hnrnpk binding, H3K4me1 binding, H3K4me3 binding and HiC profile of mice limb bud cells with or without Hnrnpk