Hnrnpk is essential for embryonic limb bud development as a transcription activator and insulator collaborator

Proper development of limb bud relies on the concordance of various signals, otherwise limb deformities occur. We report that heterogeneous nuclear ribonucleoprotein K (hnRNPK) is essential for limb bud development. here, we knock out Hnrnpk in limb bud and exert the RNA-seq, ATAC-seq, CUT&RUN-seq, and Hi-C assay using primary limb bud cells to explore the function of Hnrnpk in limb bud development. ATAC-seq, mRNA, Ctcf binding, Hnrnpk binding, H3K4me1 binding, H3K4me3 binding and HiC profile of mice limb bud cells with or without Hnrnpk肢体芽（limb bud）的正常发育依赖于各类信号的协同调控，一旦信号失调便会引发肢体畸形。本研究证实异质性核核糖核蛋白K（heterogeneous nuclear ribonucleoprotein K，hnRNPK）对于肢体芽发育至关重要。在此研究中，我们在肢体芽中敲除Hnrnpk，并利用原代肢体芽细胞开展了RNA测序（RNA-seq）、转座酶可及性测序（ATAC-seq）、CUT&RUN测序（CUT&RUN-seq）以及染色质构象捕获测序（Hi-C）实验，以探究Hnrnpk在肢体芽发育中的功能。本数据集涵盖存在或缺失Hnrnpk的小鼠肢体芽细胞的ATAC-seq数据、mRNA表达谱、Ctcf结合谱、Hnrnpk结合谱、组蛋白H3赖氨酸4单甲基化（H3K4me1）结合谱、组蛋白H3赖氨酸4三甲基化（H3K4me3）结合谱以及Hi-C图谱数据。