27-color healthy lung flow cytometry dataset

Dataset belonging to the publication "Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs" by Baumann et al. Files are raw flow cytometry data for a healthy female murine lung, stained with a 27-color antibody panel (see Figure 4 and Supplementary Table 2 in the paper). The folder ("raw") contains two subfolders, with corresponding reference single stains and lung samples. In the reference sample folder, the files are either beads or cells, corresponding to what we used for data unmixing in the manuscript. Additionally, it contains the single AF signatures used for unmixing (AF 5, 13, and 35). In the samples folder, FS is the fully stained sample. FMO denotes Fluorochrome Minus One controls. For all questions, please refer to the contact linked in the paper.本数据集隶属于Baumann等人发表的论文《用于小鼠肺部深度免疫表型分析的优化全光谱流式细胞术组合》（Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs）。 本数据集包含健康雌性小鼠肺部的原始流式细胞术数据，所有样本均采用27色抗体组合进行染色（详见论文中的图4与补充表2）。 "raw"文件夹包含两个子文件夹，分别对应参照单染样本与肺部样本。 在参照样本子文件夹中，文件分为微球与细胞两类，对应论文中用于数据解混的实验材料。 该子文件夹还包含用于数据解混的单AF特征谱（AF 5、13与35）。 在样本子文件夹中，FS代表全染色样本，FMO则代表荧光素减一对照（Fluorochrome Minus One）。 如有任何疑问，请参照论文中提供的联系方式。