Transcriptomic alterations in cortical astrocytes following the development of post-traumatic epilepsy

We aimed to identify molecular signatures of astrocytes isolated from the cortex in sham or CCI injured mice with or without spontaneous recurrent seizures Utilizing the murine unilateral focal control cortical impact (CCI) injury, we evaluated seizure onset using 24/7 EEG video analysis at 2-4 months post-injury. Isolation of region-specific astrocytes and RNA-seq was performed to determine differential gene expression in PTE+ vs. PTE- that may comport with the epileptogenic process