Global mapping of ribonucleotides embedded in DNA. Saccharomyces cerevisiae

Capture and massively parallel DNA sequencing of ribonucleotides embedded in S. cerevisiae genomic DNA Overall design: We developed a new method to map the positions of ribonucleotides embedded in DNA using the unique specificity of A. thaliana tRNA ligase. Ribonucleotides were generated in budding yeasts of different genetic backgrounds and mapped to single nucleotide resolution using the new method.