CIL:12935, Rattus rattus, glandular epithelial cell, milk secreting cell, mammary alveolar cell. In Cell Image Library

Immunoelectron microscopy of mammary gland tissue from lactating rats investigating potential structural interactions between mature lipid droplets, as indicated by peripheral labeling with adipophilin (smaller gold particles) and elements of the trans-Golgi network, identified by TGN38 (a transmembrane trans-Golgi protein) staining (larger gold particles). Note that trans‐Golgi cisternae in lactating mammary cells contain large amounts of casein and other milk proteins and are grossly expanded due to the osmolarity of the secreted fluid.本数据集针对泌乳大鼠的乳腺组织开展免疫电子显微镜术（Immunoelectron microscopy），旨在探究成熟脂滴之间潜在的结构相互作用：实验以脂滴包被蛋白（adipophilin）对脂滴外周进行标记（对应更小尺寸的金颗粒），以TGN38——一种跨膜反式高尔基体蛋白（transmembrane trans-Golgi protein）——进行染色以标识反式高尔基体网络（trans-Golgi network, TGN）的组分，其标记信号对应更大尺寸的金颗粒。需注意，泌乳乳腺细胞内的反式高尔基潴泡（trans-Golgi cisternae）含有大量酪蛋白（casein）及其他乳蛋白，且因分泌液的渗透压作用而发生显著扩张。