CRISPR/Cas9 editing of Primary Bovine Mammary Epithelial Cells
收藏NIAID Data Ecosystem2026-03-13 收录
下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA862600
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资源简介：
This project assessed four pbMEC lines recovered from pregnant cows. we describe an optimised method for preferentially selecting CRISPR-Cas9 edited cells conferring a knock out of DGAT1, using fluorescence activated cell sorting (FACS). Editing efficiency was determined by minION sequencing of a PCR amplicon of the edited region. The combination of these techniques facilitate the use of pbMECs as a model to investigate the effects of gene introgressions and genetic variation in lactating mammary tissue.
创建时间：
2022-07-26
