Genome assemblies and annotation of <i>Verpa bohemica </i>and <i>Verpa conica</i>

We constructed subchromosomal genome assemblies for six strains of Verpa combing Nanopore and Illumina sequencing technologies. The average assembly sizes were 44.38 Mb for V. bohemica and 45.40 Mb for V. conica. Additionally, Hi-C data were used to anchor V. bohemica strain 21108 and V. conica strain 21120 to chromosomes 26 and 25, respectively. Benchmarking Universal Single-Copy Orthologue (BUSCO) analysis revealed that the completeness of the final assemblies exceeds 95%. Following the assembly and quality assessments, we annotated protein-coding genes and analyzed the phylogenetic relationships of the genus Verpa. We observed the presence of multiple accessory chromosomes in Verpa, suggesting the complexity of their chromosomal evolution. Furthermore, trough analyses of expaned gene families, along with the detecting of carbohydrate-active enzymes (CAZymes), we conducted a preliminary exploration of the genetic basis underlying the saprotrophic nutritional strategy of Verpa.