De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha

Key message MADS-box genes family may play important roles in the flower sex determination in Excoecaria agallocha. A total of 73 MADS-box genes were identified in E. agallocha. De novo transcriptome assembly analysis suggested that AP1/FUL, AP3/PI, AGL104 and SOC1 plays potential roles in E. agallocha flower sex determination. Appendix Figure S1. The length distribution of transcripts (a) and unigenes (b). The inserts show the frequency distribution of the transcript length (a) and of the unigene length (b). Figure S2. Pearson correlation between samples. Figure S3. The distribution of SSRs in E. agallocha. The insert shown the distribution of the total number of SSRs in mono-, di-, tri-, tetra-, penta- and hexa-nucleotide repeats. Figure S4. The overview of E. agallocha transcriptome assembly and the characteristics of the homology search of unigenes. (a) e-value distributions of the best BLAST hits for each unigene against the NR database. (b) similarity distribution of the best BLAST hits for each unigene against the NR database. (c) venn diagram showing the BLAST searches of the E. agallocha transcriptome against the five public databases. (d) species distribution of the best BLAST hit for each unigene against the NR database. Figure S5. Gene ontology distributions for the transcriptome of four samples in E. agallocha. Main functional categories of the transcriptome related to plant physiology of the biological process, cellular component, and molecular function. The abscissas show the number of unigenes, and one unigene may be associated with different GO terms. Figure S6. The KEGG pathway for the transcriptome of four samples in E. agallocha. The unigenes were divided into five clusters according to KEGG metabolism pathways, A: Cellular processes, B: Environmental information processing, C: Genetic information processing, D: Metabolism, E: Organismal systems. Figure S7. Expression pattern validation of 14 selected DEGs in the RNA-Seq analysis by qRT-PCR in E. agallocha. Table S1. Classification of MADS-box genes in E. agallocha. Table S2. The primer sequences of MADS-box genes by qRT-PCR analysis. Table S3. Overview of the sequencing data of E. agallocha transcriptome. Table S4. The summary of SSR in the total of unigenes. Table S5. KEGG annotation of nonredundant unigenes in E. agallocha. Table S6. KEGG annotation of DEGs of E. agallocha in Ea_ff vs Ea_mf (a), Ea_ff vs Ea_fL (b), Ea_mf vs Ea_mL (c) and Ea_fLvs Ea_mL (d) group. Table S7. The Raw data of qRT-PCR analysis. Table S8. Results of DEGs in each sample were identified by calculating the expression levels of each transcript using the transcripts per million reads method. Reference Zhou Y, Hao L, Huang L, Tang X, Zhuo D, Wang LY, Zhang Y (2022) De novo transcriptome assembly reveals characteristics of flower sex determination of Excoecaria agallocha. Annals of Forest Science.