RNA sequencing of nas-38(ok4307), nas-38(tm2655) and wild type C. elegans during L4 lethargus using NextSeq500

The goal of this study was to investigate the differential gene expression between wild types and different nas-38 mutants during lethargus. Extremely prolonged lethargus in nas-38(ok3407) mutants and slightly shortened lethargus in nas-38(tm2655) was observed before. Per condition, three biological replicates were prepared. RNA isolation, library preparation, sequencing and fold change analysis was done by IMGM Laboratories GmbH.