Chisholm Lake sedimentary fish metabarcoding DNA sequence reads, 2019 - 2023

We sequenced polymerase chain reaction (PCR) amplicons targeting a segment of the 12S ribosomal DNA gene in vertebrates from DNA isolated from sections of a sediment core from Chisholm Lake in interior Alaska. The primary aim of this sequencing effort was to add to our understanding of the evolution of fish faunas in Beringian regions impacted by glacial cycles. This dataset consists of unprocessed output from amplicon paired end short read sequencing on an Illumina 'sequencing-by-synthesis' platform. Analyses of these sequences are being incorporated into a broader effort to examine post-glacial Beringian biotas and ecologies.