Supplemental Material for Riles and Fay, 2018

Data availability statement: All strains and constructs
are available upon request to the corresponding author. Supplemental Table 1: Strains used in this study. Supplemental
Table 2: Recombinant strain barcode indices, sequencing and phenotypes used for
QTL mapping. Supplemental Table 3: Primers used in this study. Supplemental
Table 4: Summary of candidate genes tested for two QTL regions. Supplemental
Table 5: Genes tested for complementation using a MoBY and/or a hemizygosity
test. Supplemental Table 6: Colony size measurements using ImageJ for HN6 X KO
Hemizygotes on Chromosome 14. Supplementary Figure 1: Ethanol and heat
sensitivity of control strains. Supplementary Figure 2: Logarithm of the odds
ratio (LOD) of a quantitative trait locus for heat and ethanol tolerance at
high temperature across the genome. Supplementary Figure 3: Protein alignment
of HN6, Oak and S288c for SEC24 and PSD1. Supplementary Figure 4: Phenotypes of resistant wild yeast
strains, Oak, Wine and S288c, containing high copy Oak allele plasmids grown at
40o. Data File S1. Genotypes and phenotypes of recombinant strains
used for QTL mapping. The raw data used to call genotypes for QTL mapping has
been deposited into NCBI's SRA under BioProject: PRJNA480857.

数据可用性声明：所有菌株和构建均可根据对应作者的要求提供。补充表1：本研究中使用的菌株。补充表2：用于QTL作图的重构菌株条形码索引、测序和表型。补充表3：本研究中使用的引物。补充表4：对两个QTL区域进行测试的候选基因总结。补充表5：使用MoBY和/或杂合性测试进行互补性测试的基因。补充表6：使用ImageJ对14号染色体上的HN6 X KO杂合子进行菌落大小测量的结果。补充图1：对照菌株对乙醇和热敏感性的分析。补充图2：全基因组中高温下耐热和耐乙醇的量化性状位点（QTL）的似然比（LOD）的对数。补充图3：HN6、Oak和S288c的SEC24和PSD1蛋白质比对。补充图4：在高40°C下生长的携带高拷贝Oak等位基因质粒的耐性野生酵母菌株Oak、Wine和S288c的表型。数据文件S1：用于QTL作图的重构菌株的基因型和表型。用于QTL作图基因型调用所使用的原始数据已存档至NCBI的SRA，项目编号为：PRJNA480857。