Bacterial raw data of nanoplastics threatment in the soil-root habitats of Torreya grandis

Micro/nano plastic is a new kind of pollutant, which can be retained in the soil environment for a long time with effects on the soil ecosystem. Using 16S/ITS rRNA gene sequencing and untargeted metabolomics, we analyzed microbiomes and metabolomes of different Torreya grandis soil-root microbial habitats after nanoplastics (polystyrene) treatment for three month. Our results showed that nanoplastics can significantly change the composition and function of soil microbial communities. Nanoplastics treatment reduced the relative abundance of Ascomycota in rhizosphere and bulk soils, but enhanced their abundance in the root endophytic samples. Co-occurrence network analysis of the T. grandis root endosphere revealed the bacterial richness and network complexity increased after nanoplastic treatment, while the fungal richness and network complexity decreased. KEGG analysis indicated that nanoplastics lead to an increased capacity for the degradation of some biological macromolecules and changes to the NAD/NADP-NADH/NADPH metabolic pathway, indicating that nanoplastics affect the transformation of soil metabolites, elements and energy. Nanoplastics affected lipid metabolism in bulk soil, and affected the flavonoid and terpenoid synthesis pathways in rhizospheric soil. Amino acid and energy metabolism were altered by the presence of nanoplastics in the T. grandis roots. Our research provides new insights for the assessment of soil health and forest micro/nano plastics pollution.