Effects of GSTM1 knockdown on astrocyte transcriptional responses to TNF-alpha stimulation

To investigate the role of GSTM1, we knocked down GSTM1 expression in primary mouse astrocytes using lenvirtus vector expressing Gstm1 shRNAmir and then exposed them to TNF-alpha. Non-silencing shRNAmir and non-stimulation served as shRNA and treatment controls, respectively. Comparative gene expression profiling analysis of RNA-seq data for astrocytes with shGstm1 and shControl in 1) no-stimulation, and 2) TNF-alpha stimulation (6 h, 50 ng/ml).